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KMID : 0525720120170010019
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Journal of Chitin and Chitosan
2012 Volume.17 No. 1 p.19 ~ p.23
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Effect of Chitosan Oligosaccharide on Metastatic Activities in MCF-7 Estrogen-Dependent Human Breast Cancer Cells
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Shin Jin-Sun
Lee Kyu-Shik
Nam Kyung-Soo
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Abstract
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We investigated the effect of chitosan oligosaccharide (COS-L, MW < 1,000 Da) on metastatic activities in MCF-7 estrogendependent human breast cancer cells. In a concentration range of 0.1 to 5 mg/mL, aromatase transcription was significantly down-regulated by COS-L. In gelatin zymographic analysis, matrix metalloproteinase-9 (MMP-9) activity induced by phorbol- 12-myristate-13-acetate (TPA) was critically inhibited by COS-L in a dose-dependent manner. In contrast, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) transcriptions was enhanced by COS-L but not changed TIMP-2. Therefore, the results suggest that COS-L should decrease the metastatic potential in MCF-7 estrogen-dependent human breast cancer cells through the decrease of aromatase transcription and MMP-9 activity and the increase of TMP-1 expression. In conclusion, present investigation provides critical information that COS-L may diminish the risk of mortality and recurrence caused by metastasis in estrogen-dependent breast cancer.
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KEYWORD
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Chitosan oligosaccharide, Breast cancer, Aromatase, Matrix metalloproteinase-9, Tissue inhibitor of metalloproteinases, Metastasis
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